Analysis of the 9p21.3 sequence associated with coronary artery disease
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IMAGE: The diagram showing a general scheme of TAR cloning of a region of interest (in red) from total genomic DNA (in blue) with a linearized TAR vector containing a yeast… view more 

Credit: Natalay Kouprina, email: kouprinn@mail.nih.gov

Before a conclusive link between the SDs and the cardiovascular diseases can be made, further analysis is required on the CAD interval in more patients with coronary artery disease and in the human population, using the TAR cloning technique in combination with qPCR or Droplet digital PCR developed in this work.

The research team said that “in the past years, an emerging group of human genetic diseases have been described that result from DNA rearrangements rather than from single nucleotide changes. In humans, copy-number variations of such repeats have been implicated in common traits such as neuropathy, hypertension, color blindness, infertility, and behavioral traits, including autism and schizophrenia, as well as disease susceptibility to HIV, lupus nephritis, psoriasis and Parkinsons disease.”

In this work, they focused on the analysis of structural variations within the CAD interval sequence using different approaches, including re-isolation of this region by the transformation-associated recombination cloning technique.

Here, they used TAR cloning for isolation of the CAD

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