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IMAGE: A Z-stack image was obtained after staining nuclei with Hoechst using confocal microscopy at 5 micron intervals and then assembled using ImageJ software. view more 

Credit: Shurong Hou, Ph.D.

A multidisciplinary team of scientists share recent advancements in innovative in-vitro cancer biology methods for screening drug-like molecules in cancer tissue relevant models in a new report published online ahead-of-print at SLAS Discovery. Entitled “Advanced Development of Primary Pancreatic Organoid Tumor Models for High-Throughput Phenotypic Drug Screening,” the report can be accessed for free.

The authors — Senior Scientific Directors Timothy Spicer and Louis Scampavia and Post-Doctoral Associate Shurong Hou at Scripps Florida in collaboration with Cold Spring Harbor Laboratory, Greiner Bio-One, Nano3D Biosciences, Inc., University of Texas Health Science Center at Houston, and the Dana-Farber Cancer Institute — illustrate how a magnetic nanoparticle assembly approach is used to increase throughput dramatically while reducing costs. This technology combines specialized high-density microtiter plates formulated with an ultra-low attachment surface along with gold nanoparticles (nanoshuttles), which are used to label cancer cells in-vitro. Once labeled, a magnetic driver quickly pulls the cells into a 3D spheroid or organoid structure. This 3D structure is retained and drug-like molecules can then be added,

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